Golgi-associated tethers capture vesicles from specific membrane recycling pathways.

Golgi-associated tethers in Saccharomyces cerevisiae capture vesicles from specific intra-Golgi recycling pathways, with GARP tethering vesicles dependent on AP-1 and Ent5 adaptors.

• Why it matters: Understanding how Golgi proteins are recycled is crucial for elucidating Golgi function and dynamics, yet the specific tethers and pathways involved remain incompletely characterized.
• What they did: The study examined three recycling pathways by identifying tether presence on maturing cisternae and ectopically localizing tethers, revealing that Sgm1 captures COPI-dependent intra-Golgi vesicles at an intermediate stage, while Imh1 and GARP tether vesicles dependent on AP-1 and Ent5 at a late stage.
• The result: Findings highlight GARP’s role in intra-Golgi recycling and clarify that Imh1 tethers vesicles from prevacuolar endosomes, advancing the mechanistic understanding of Golgi vesicle capture and recycling pathways.