Multi-immersion oblique plane microscope (miOPM): a reconfigurable platform for high-resolution light-sheet fluorescence microscopy.

miOPM achieves high-resolution, diffraction-limited imaging across a refractive index range of 1.33 to 1.51, supporting diverse biological specimens and scales.

• Why it matters: Current LSFM variants are narrowly optimized, limiting their broad applicability and accessibility for different samples and imaging regimes.
• What they did: The team developed miOPM, a reconfigurable platform that seamlessly switches between oil, water, and air objectives, compatible with standard mounting and clearing protocols, enabling high-throughput 3D imaging.
• The result: This adaptable system expands the usability of LSFM, potentially democratizing advanced 3D imaging for various biological research applications.