Multi-omics atlas of chronic exercise.

Exercise induces tissue-specific molecular remodeling with the largest transcriptomic changes observed in skeletal muscle in mice.

• Why it matters: Understanding the molecular effects of exercise across different organs, sexes, and circadian phases can inform strategies to improve health and reduce mortality.
• What they did: The study involved 8 weeks of voluntary wheel running in mice, with multi-omics analyses (RNA sequencing, metabolomics, proteomics, methylation sequencing) performed on 22 tissues and plasma during active and inactive phases, including cell-type resolution.
• The result: Exercise caused widespread, tissue-specific changes, especially during active phases, with sex-biased epigenetic responses and conserved signatures identified across mammalian species, enabling deeper insight into exercise’s molecular impact.