Mycn facilitates hematopoietic stem and progenitor cell generation from endothelial cells by inhibiting adhesion signaling.

Endothelial-specific deletion of Mycn, but not Myc, impairs hematopoietic stem and progenitor cell formation in mouse embryos by attenuating the HEC-to-HSPC transition.

• Why it matters: Mycn is highly expressed in hematopoietic stem cell–primed hemogenic endothelial cells, but its role and mechanism in the endothelial-to-hematopoietic transition remain unclear.
• What they did: The study used endothelial-specific Mycn or Myc deletion in mouse embryos, single-cell transcriptomics, functional assays, and a mosaic analysis strategy to map deletion states. It found Mycn-deficient HECs upregulate adhesion pathways, and focal adhesion kinase inhibition rescues HSPC production in explant cultures.
• The result: Mycn promotes HSPC generation from endothelial cells by suppressing adhesion signaling, specifically during the HEC-to-HSPC transition in embryos and explant cultures.

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