Deep phenomics of African green monkey and Baboon PBMCs using a 30-marker spectral flow cytometry panel

A custom 30-parameter spectral flow cytometry panel enables deep profiling of major immune cell subsets and T-cell functional markers in African green monkey and baboon PBMCs.

• Why it matters: Existing deep phenomics panels for human T cells are not widely available for non-human primates, limiting immune monitoring for vaccine and infection studies in AGMs and baboons.
• What they did: The authors built and validated a 30-marker spectral flow cytometry panel for AGM or baboon PBMCs, measuring B cells, NK cells, DCs, monocytes, plus Th1 (IFN{gamma}, TNF, IL-2), Th2 (IL-4/5/13), Th17 (IL-17), AIMs (CD69, CD134/OX40, CD137/4-1BB), and FoxP3+ Tregs in steady state and mitogen-stimulated conditions.
• The result: The validated AIMs (CD69, CD134/OX40, CD137/4-1BB) support exploratory analysis of how cytokines, activation markers, or Tregs relate to host immunity after infection or vaccination, with customization for specific research needs.

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