Core MCC of Arabidopsis thaliana: structural dissection provides new insights into Mad2, BubR1 and CDC20

Structural dissection of the core mitotic checkpoint complex in Arabidopsis thaliana identifies 53 nonredundant crosslinks among AtCDC20, AtBubR1, and AtMad2, revealing domain-specific interaction sites.

• Why it matters: Plant SAC mechanisms remain poorly defined, and the molecular wiring of the core MCC in A. thaliana is scarce despite progress on plant cell-cycle protein interactions.
• What they did: Using bigBac, chemical crosslinking, mass spectrometry, and structural analysis, the study mapped PPIs among AtCDC20, AtBubR1 (BMF2), and AtMad2, including interaction hierarchy and Mad2 tetramer features.
• The result: The work finds multiple binding sites across domains (including a new KET box in AtBubR1 and Horma domain in AtMad2), no PPIs for AtBub1 (BMF1) or AtBub3:1, and provides a structural basis for core MCC formation in A. thaliana.

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