The Src family kinases Lck and Fyn promote transendothelial migration of T cells

Lck and Fyn are required for efficient T cell transmigration across an inflamed endothelial barrier, with Lck also needed for PI3K signaling downstream of LFA-1.

• Why it matters: T cells must cross endothelial barriers to enter peripheral tissues, but the specific signaling events that enable migration and transmigration remain incompletely defined.
• What they did: Primary T cells lacking Lck, Fyn, or both were tested for random migration on ICAM-1 and for transmigration across inflamed endothelium, alongside signaling analyses of PI3K activity and p85 interactions.
• The result: Lck deficiency impaired PI3K signaling downstream of LFA-1 and disrupted p85 binding to tyrosine-phosphorylated partners, while both kinases were needed for transmigration; an Lck inhibitor reduced PI3K signaling and transmigration.

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