bioRxivJClub
Genome assembly and characterization of two Brazilian soybean cultivars contrasting for stink bug resistance
bioRxiv · · Preprint · Open access
Godoy Filho, Viana + 4 more
Abstract ↗AI summary
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Chromosome-level de novo assemblies for Brazilian soybean cultivars IAC-100 (stink bug resistant) and CD-215 (susceptible) span 1.057–1.061 Gb with scaffold N50 of 53.6–54.2 Mb.
- Why it matters: Tropical soybean germplasm has limited genomic resources, with only one Brazilian cultivar assembly available in public databases, limiting studies of insect resistance.
- What they did: Using 10x Genomics linked reads from high molecular weight DNA, the team built Supernova assemblies, anchored them to Williams 82 with RaGOO, and corrected structural errors with REAPR.
- The result: BUSCO recovery exceeded 97% and homology projection produced 46,774 and 46,620 gene models; proteome comparison found 2,002 divergent isoforms across 1,427 loci, supporting insect-resistance research.
The findingWhy it mattersWhat they didThe result
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