SPACE-Tag Enables High-Throughput Spatial Chromatin Profiling With CUT&Tag

SPACE-Tag, a spatial CUT&Tag framework in mouse brain, enables parallel profiling of five histone modifications and transcriptome across adult lifespan, producing the largest spatial multimodal dataset to date.

• Why it matters: Spatial epigenomics is constrained by chromatin accessibility and throughput, limiting how well chromatin states can be mapped at scale and integrated with spatial transcriptomics.
• What they did: The method uses T7-based linear amplification to convert epigenetic marks-associated DNA into polyadenylated RNA, then integrates with standard spatial transcriptomic workflows.
• The result: In aging mouse brain, fiber tracts show widespread transcriptional downregulation often without nearby chromatin state transitions, while isocortex displays coordinated chromatin–transcriptome changes, including an H3K4me1 peak module increasing with age.

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