Integrative identification of Tetratostichococcus sp. CCAH0201 (Trebouxiophyceae, Chlorophyta) from Rhizophora leaves in the Can Gio Mangrove Forest, Vietnam

Tetratostichococcus sp. CCAH0201 from Rhizophora leaves in Vietnam’s Can Gio Mangrove Forest is identified as a lineage close to P1 using SSU rDNA, ITS, and rbcL.

• Why it matters: Mangrove leaf microalgae remain under-characterized, and reliable identification needs multiple genetic markers linked to morphology and sequence differences.
• What they did: The strain was examined by light microscopy and three molecular markers (SSU rDNA, ITS, rbcL) using homologous phylogenetic windows of 550, 711, and 1,170 nucleotides.
• The result: CCAH0201 shares SSU 3-prime sequence with CCAH0201, P1, and Tetratostichococcus jenerensis SAG 2138, but differs from P1 at 22/711 ITS and 4/1,170 rbcL sites, with ITS2 showing one strict CBC and additional changes.

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