Sequential pathways preserve kinetochore integrity in meiosis

In budding yeast meiosis, all three centromere-to-outer-kinetochore connections—Ctf19cCCAN, Mif2CENP-C, and Cse4CENP-A END binding by Okp1CENP-Q-Ame1CENP-U—are required to preserve kinetochore integrity.

• Why it matters: It remains unclear how kinetochores keep their structural integrity while being remodelled during meiosis, despite multiple centromere-outer kinetochore links.
• What they did: The study mapped three distinct pathways from centromeric chromatin to the outer kinetochore, tested their redundancy and timing across meiotic stages, and assessed roles of Ipl1Aurora B and Cse4CENP-A N-terminal domain phosphomimetic mutations.
• The result: Ipl1Aurora B maintains kinetochore integrity independently of Ctf19cCCAN via an outer-kinetochore-associated pool, and Cse4CENP-A phosphomimetic mutations rescue Ipl1Aurora B depletion but worsen defects when Ctf19cCCAN is absent.

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