hAM-pe soluble extract increases human keratinocyte HaCaT cell-line mobility in scratch assays

hAM-pe soluble extract reduces the average time for HaCaT keratinocytes to completely close a scratch-wound, with automated segmentation supporting robust treatment comparisons.

• Why it matters: Wound healing models need quantitative ways to compare collective cell displacement, and it remains unclear whether faster closure reflects individual speed or altered motion dynamics.
• What they did: The study used scratch assays on HaCaT cells with automated wound-edge segmentation, linear fitting for comparisons, and single-cell tracking plus mean squared displacement and active Brownian particle simulations.
• The result: Individual cells under treatment did not increase speed enough to explain closure, while simulations and mean squared displacement support increased persistence of cellular motion as the key mechanism.

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