Evaluating the Identifiability of Temporal Delay and Response Attenuation in Endometrial Decidualization

In GSE246591, a candidate 24-hour temporal shift filter removed 30.9–38.3% of initially dynamic endometrial stromal features, showing temporal delay and attenuation can be hard to separate.

• Why it matters: Endometriosis-linked impaired decidualization can look similar across time points, because delayed activation and reduced response amplitude can produce overlapping expression patterns with different biological meanings.
• What they did: The study used an identifiability-aware longitudinal transcriptomics framework with constrained amplitude-only versus amplitude-plus-delay models, analytical equivalence examples, and simulation-based distinguishability, then tested held-out time points and culture-level sensitivity.
• The result: A two-component diagnostic indicated small projection residuals can reflect either loss of observable dynamics or affine response-shape similarity, and independent in vivo score changes in GSE51981 (phase estimate 0.155, 95% CI 0.222 to 0.088) and GSE6364 (0.033, 95% CI 0.068 to 0.002) did not independently establish a temporal delay.

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