Organ scale Three-dimensional atlas of developing pelvic innervation for the mouse lower urinary tract.

A wholemount 3D light-sheet fluorescence atlas maps mouse pelvic autonomic neuron development from mid-gestation through post-weaning, including migration routes and pelvic ganglia maturation.

• Why it matters: Pelvic autonomic neurons coordinate bladder storage and emptying, but complex genitourinary morphogenesis has limited precise 3D tracking of how these neurons form and organize.
• What they did: Intact mouse lower urinary tract tissue was imaged at single-cell resolution across fetal to post-weaning stages to follow neural crest progenitor migration, neurogenesis, and pelvic ganglia morphology.
• The result: The atlas pinpoints migration timing and routes, defines pelvic ganglion neurogenesis timing, and uncovers neuronal cell bodies outside centralized pelvic ganglia plus bladder-proximal accessory ganglia emerging with maturation.

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