Tn-Seq-based identification of genes that play a role in antimicrobial tolerance of Pseudomonas aeruginosa aggregates

Genome-wide Tn-Seq screening of thousands of clonal Pseudomonas aeruginosa aggregates identifies multiple genes and pathways linked to aggregate-associated antimicrobial tolerance, including class-specific and class-independent factors.

• Why it matters: Biofilm and aggregate infections persist because of antibiotic tolerance, but the mechanisms and which known factors matter most remain unclear.
• What they did: The study used a Tn-Seq workflow to generate and test thousands of single-transposon mutant aggregates, then compared their relative tolerance across antimicrobial treatments.
• The result: Most identified factors were linked to tolerance to specific antibiotics, while some were linked to tolerance across antibiotic classes, enabling adaptation of the approach to other species and stressors.

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