Rapid Electro-optic Two-photon Bessel Light Sheet Fluorescence Lifetime Imaging for Tissue Volume Interrogation

Rapid electro-optic two-photon Bessel light sheet FLIM (TPLS-EO-FLIM) achieves sub-nanosecond lifetime resolution and accurate images in 10 ms exposures with a few hundred photons per pixel.

• Why it matters: Multiphoton FLIM needs high detection efficiency for deep, low-signal tissue volumes, while fast acquisition and reduced phototoxicity are critical for live functional imaging.
• What they did: The method uses scanning two-photon Bessel light sheet excitation with an electro-optic modulation detection module and an sCMOS detector to enable wide-field, time-resolved volume acquisition.
• The result: In live Danio rerio embryos, it records cardiac cycle dynamics at 50 frames per second and quantifies heart rate, ventricle/atrium areas, valve dynamics, and fractional area change.

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