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Influence of antibody-antigen interaction on fluorescence of Rhodamine 6G conjugated to the antibodies measured in human serum
bioRxiv · · Preprint · Open access
Thiyagaraj, Del Re + 4 more
Abstract ↗AI summary
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Samples with 100 nM trastuzumab (TRAS) in human serum showed the largest spectral changes in Rhodamine 6G emission energy and emission width upon interaction with HER2, indicating nonlinearity across antibody concentration.
- Why it matters: Antibody-antigen and antibody-antibody interactions can shift dye energetics, but how these spectral reporters behave in physiologically relevant media is unclear.
- What they did: Rhodamine 6G–conjugated monoclonal antibodies (TRAS and pertuzumab) were tested in HBS-8 and in diluted and undiluted human serum, varying antibody concentration and, for TRAS, recombinant human HER2 extracellular domain concentration.
- The result: Antibody interactions produced substantial changes in emission energy and emission width that depended on serum composition, and binding partners could be detected under physiologically relevant conditions.
The findingWhy it mattersWhat they didThe result
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- Open access