Exclusion from DNA double-strand breaks is a pervasive feature of the hnRNP family

Thirteen of 14 hnRNP family members are excluded from laser-induced DNA double-strand break sites, with hnRNPH1 showing sustained exclusion for several hours.

• Why it matters: Individual hnRNPs have been linked to the DSB response, but their collective behavior at damaged chromatin was unclear because prior work examined proteins one at a time under different conditions.
• What they did: The core hnRNP family was compared side by side using laser microirradiation and live-cell imaging; hnRNPH1 exclusion was tested for dependence on signaling, transcription, and the GYR low-complexity domain.
• The result: Exclusion predominates over accumulation, persists for hours, occurs at nuclease-induced breaks at a defined locus, and requires the GYR domain with RNA binding via three quasi-RNA recognition motifs, selectively impairing 53BP1 accumulation.

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