KDM6A is enzymatically active at the Golgi apparatus where it is necessary for maintenance of Golgi apparatus morphology

KDM6A is enzymatically active at the Golgi apparatus and is necessary for maintaining Golgi morphology in MCF10A breast cells, with activity linked to its JmjC domain.

• Why it matters: KDM6A is known for transcriptional regulation and cancer relevance, but its subcellular function beyond the nucleus—especially during TGF-β–related changes—was unclear.
• What they did: Using immunofluorescence and LC-MS/MS, the study found KDM6A lost from the nucleus but retained in the Golgi, and biochemically separated membranous fractions showed JmjC-containing enzymatic activity; knockdown and TGF-β similarly altered Golgi morphology.
• The result: The results support a context-dependent model where KDM6A maintains Golgi apparatus morphology and can act on non-nuclear substrates, implying functional roles outside transcription.

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