GENOME ASSEMBLIES FOR 28 PHAEOSPHAERIACEAE ISOLATES FROM CANADA

Whole genome sequencing and assembly of 28 Canadian Phaeosphaeriaceae isolates produced mostly very complete genomes with BUSCO >99% and ONT-derived assemblies with very few contigs.

• Why it matters: Glume blotch and Septoria Tritici Blotch (STB) are destructive crop diseases, and recent taxonomic revisions require better genomic resources to clarify diversity and relationships among reclassified fungi.
• What they did: The team combined Oxford Nanopore Technologies (ONT) long reads with Illumina short reads, assessed completeness with BUSCO, and compared isolates to 2,668 Dothideomycete genomes from NCBI using NUCmer whole-genome alignments.
• The result: Twenty-seven isolates clustered discretely with Parastagonospora nodorum or Parastagonospora pseudonodorum, while one genome had no close NCBI match and is hypothesized to be the first assembly of Phaeosphaeria pontiformis.

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