Deep phenomics of human PBMCs using a 31-marker spectral flow cytometry panel

A 31-parameter spectral flow cytometry panel enables simultaneous profiling of functional cytokines, AIMs (CD69, CD134/OX40, CD137/4-1BB), and FoxP3+ Tregs in human PBMCs.

• Why it matters: Existing approaches separately track intracellular cytokines or AIMs, making it difficult to deep-phenotype functional cytokine signatures alongside activation markers and the nuclear FoxP3+ Treg compartment.
• What they did: The authors built and validated a 31-marker panel that profiles major immune subsets and T cell states, measuring Th1 (IFN{gamma}, TNF, IL-2), Th2 (IL-4/5/13), and Th17 (IL-17) signals plus AIMs and FoxP3+ Tregs in steady state and mitogen-stimulated PBMCs.
• The result: Re-validation after peptide stimulation supports mapping antigen-specific Th1/2/17 distributions and AIMs alongside FoxP3+ Tregs, enabling detailed T cell immune profiling for exploratory studies after infection or vaccination.

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