Differential Gene Expression and Functional Enrichment Analysis of Breast Tumor and Normal Tissue RNA-seq Data: A Reanalysis of GEO Dataset GSE210787

Reanalysis of GEO GSE210787 (6 breast tumors vs 6 normal tissues) identified 120 DEGs, with 69 upregulated and 51 downregulated, but no KEGG pathway met adjusted significance.

• Why it matters: Tumor–normal RNA-seq comparisons can reveal biology, yet reanalysis is needed to verify DEG lists and whether pathway enrichment truly reaches FDR thresholds.
• What they did: Using SRA Toolkit, FastQC/MultiQC, fastp trimming, STAR alignment to GRCh38.p14, featureCounts quantification, and DESeq2 in Galaxy, the study applied adjusted p-value < 0.05 and |log2 fold change| ≥ 1.
• The result: GO enrichment found the upregulated “cell periphery” term (GO:0071944) with P ≈ 0.017585 and 29 overlapping genes, while downregulated GO terms and KEGG pathways were not significant after multiple testing.

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