Carboxymethyl Dextran Coated Iron Oxide Magnetic Nanoparticles as Vehicles for CRISPR Ribonucleoproteins

CMD-coated iron oxide magnetic nanoparticles form stable nanocomplexes with Cas9 and Cas12a RNPs, enabling functional genome editing in HEK-293T cells and primary human dermal fibroblasts.

• Why it matters: Intracellular delivery of CRISPR ribonucleoproteins is a major barrier for genome editing, and magnetic nanoparticle carriers for functional RNPs remain comparatively underexplored.
• What they did: The team assembled electrostatically formed CMD-coated MNP nanocomplexes with Cas9/Cas12a RNPs, verified uptake by microscopy, tested stability in serum-supplemented medium for up to 24 hours, and assessed cytotoxicity.
• The result: Cas9 RNP nanocomplexes achieved exon 80 deletion at the COL7A1 locus in a recessive dystrophic epidermolysis bullosa (RDEB) strategy targeting c.6527insC, supporting further magnetic nanoparticle-mediated editing for inherited skin disorders.

AI summary of the abstract · may contain errors · not medical advice · read the paper.