Pho92 direct interaction with Not1 recruits the CCR4-NOT complex to m6A containing mRNAs to modulate meiosis in S. cerevisiae

Pho92 directly binds Not1 and recruits the CCR4-NOT deadenylase complex to m6A-containing mRNAs in Saccharomyces cerevisiae, enabling efficient meiotic progression.

• Why it matters: m6A is deposited only during meiosis in budding yeast, and losing Pho92 causes abnormal persistence of m6A-containing transcripts and delayed meiotic progression.
• What they did: The study combined in vivo and in vitro assays to map direct Pho92–Not1 interactions, then used structural analyses to show a conserved Pho92 motif forms an alpha-helix docking onto Not1.
• The result: Pho92–Not1 contacts are required for efficient meiotic progression, linking m6A recognition to the main deadenylase to shape the meiotic transcriptome.

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