Validated backbone assignments for natural-abundance 1H,15N spectra of commercial hen egg white lysozyme and example applications thereof

Backbone 1H,15N chemical shifts for commercial hen egg white lysozyme (HEWL) at pH 3.8 and 308 K are validated for natural-abundance spectra, enabling ready-to-use Sparky assignments.

• Why it matters: Existing HEWL assignment resources differ in sequence, experimental conditions, and nuclei, and no BMRB entry explicitly matches natural-abundance 1H,15N backbone assignments for concentrated commercial HEWL spectra.
• What they did: Backbone shifts were parsed from prior papers and checked against HSQC and SOFAST-HMQC correlation spectra, using a 3 mM natural-abundance sample on an 800 MHz magnet with a cryoprobe; pH titration transferred assignments to pH 6 at 308 K.
• The result: The assignments support applications including small-molecule titration, Het-SOFAST-HMQC hydration/dynamics studies, and use in published papers, and are provided as Sparky files plus selected spectra via WebNMR for visualization.

AI summary of the abstract · may contain errors · not medical advice · read the paper.