Multi-scale spatial transcriptomic atlas of the whole mouse spinal cord

Stereo-seq profiling of 322,827 cells across 81 registered transverse sections resolves 12 major cell classes and 94 transcriptomic subclusters across the whole adult mouse spinal cord.

• Why it matters: Molecularly defined cell-type organization across the entire spinal cord is incompletely understood, limiting how spatial gene programs and anatomical domains are linked to transcriptomic diversity.
• What they did: The study generated a genome-wide, single-cell-resolution spatial atlas of the whole adult mouse spinal cord using Stereo-seq, then mapped conserved anatomical territories and coordinated spatial gene-expression modules.
• The result: Oligodendrocyte subtypes show distinct, reproducible laminar organization within spinal cord white matter across spinal levels, enabling hierarchical spatial domains that recapitulate canonical anatomy while revealing finer subdivisions.