Screening and evaluation of medicine food homology herbal combinations for ameliorating chemotherapy induced ovarian aging under an integrated multi-strategy framework

Astragalus ginseng (Huangqi Renshen) herb pair (HXF) is identified as a core MFH combination that ameliorates chemotherapy-induced ovarian aging, validated in a D-gal KGN model and cyclophosphamide-treated rats.

• Why it matters: Ovarian aging threatens reproductive health, yet MFH formula screening often lacks mechanistic evidence and a systematic, target-guided discovery strategy.
• What they did: The authors integrated GEO single-cell transcriptomics, human-use evidence mining, and UPLC/Q TOF MS profiling, then used pretrained protein representation models (ESM T5) with molecular dynamics and SPR to predict compound–target affinity.
• The result: Key targets included CYP19A1, SERPINE1, and MDK; HXF restored CYP19A1-linked steroidogenesis, downregulated SERPINE1/IL18 inflammation and matrix remodeling, and reduced MDK NCL colocalization signals in vitro and in vivo.