Raman spectra identify vancomycin-resistant phenotypes and their transcriptomic features in Staphylococcus aureus.

Dimension-reduced Raman spectra from single Staphylococcus aureus cells determine vancomycin resistance levels across multiple MRSA strains and predict their transcriptomic profiles.

• Why it matters: Rapidly assessing vancomycin-non-susceptible MRSA resistance and linked molecular features is needed to prevent spread, but broad resistance diversity has limited fast characterization.
• What they did: The study measured Raman spectra from single cells and used simple linear regression to predict transcriptome profiles of different strains from those spectra.
• The result: Raman-transcriptome mapping in the reduced Raman space characterizes gene groups tied to phenotypes and identifies a strain with higher phenotypic heterogeneity, validated by single-cell growth analysis.

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