Sustainable 2-keto-L-gulonic acid biosynthesis via single-strain one-step fermentation.

Engineered single-strain one-step fermentation using Gluconobacter oxydans is a promising route for sustainable 2-keto-L-gulonic acid production, but L-sorbose-to-2-KLG conversion remains a key bottleneck.

• Why it matters: Current vitamin C manufacturing depends on multi-strain, multi-step fermentation followed by chemical lactonization, which suffers from long cycles, unstable mixed cultures, and difficult control.
• What they did: This review summarizes advances in single-strain, one-step 2-KLG systems built on Gluconobacter oxydans, emphasizing genetic tool development, metabolic engineering, and optimization of key enzymes.
• The result: The collected strategies provide a theoretical basis and technical reference to improve and industrialize greener biomanufacturing systems for vitamin C by targeting the conversion bottleneck.

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