Nucleic Acids ResJClub
scSpAS: an integrated single-cell and spatial atlas of transcript isoform regulation.
Nucleic Acids Research · · Journal Article
Chen, Liu + more
Abstract ↗AI summary
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scSpAS integrates human and mouse single-cell and spatial transcript isoform regulation, cataloging 648 000+ alternative splicing events, 432 000 ATS, and 621 000 APA sites.
- Why it matters: Regulatory layers for transcript initiation, splicing, and 3'-end processing are split across separate resources, limiting joint analysis across cell types and tissue locations in health and disease.
- What they did: The database compiles 123 standardized datasets (418 000+ cells; 82 000 spatial spots) using 5' and 3' capture scRNA-seq for ATS/APA and Smart-seq2 plus long-read data for splicing and isoforms.
- The result: scSpAS provides searchable retrieval and differential usage analyses across cell-type and spatial levels, plus regulatory annotations from CLIP-seq, ATAC-seq, and CAGE-seq with predicted splicing motifs.
The findingWhy it mattersWhat they didThe result
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