Multienzyme cascades for non-grain carbon feedstocks valorization: Recent advances and perspectives.

Intracellular multienzyme cascade catalysis enhances bioconversion efficiency for non-grain carbon feedstocks by overcoming key limitations in biomanufacturing systems.

• Why it matters: Efficient utilization of gaseous, liquid, and solid non-grain carbon sources is hindered by mass-transfer issues, thermodynamic constraints, and substrate toxicity, limiting sustainable biomanufacturing progress.
• What they did: The review analyzes mechanisms and engineering strategies of whole-cell cascade systems, proposing a universal diagnostic and design framework, and explores AI-driven predictive system reconstruction and optimization.
• The result: This work provides a unified theoretical and practical framework that enables rational design of advanced biomanufacturing platforms, facilitating tailored, efficient conversion of specific non-grain substrates.