Genome-wide discovery of cis-regulatory elements in a large genome.

Genome-wide mapping reveals conserved cis-regulatory elements in the 3.6 Gbp genome of Parhyale hawaiensis, enabling more efficient identification of functional DNA regions.

• Why it matters: Understanding cis-regulatory elements is crucial for decoding gene regulation, but their discovery is difficult in large genomes due to dispersed and non-functional DNA, limiting research progress.
• What they did: The study used bulk and single-nucleus ATAC-seq to map chromatin accessibility across tissues and cell types, and sequenced three related species to identify conserved DNA regions with low-coverage genome sequencing, all without genome assembly.
• The result: These methods successfully identified cis-regulatory elements that control gene expression, facilitating broader access to regulatory element discovery and advancing functional genomics in large genomes.