Cumulative transcription factor binding and p300-mediated histone acetylation drive enhancer activation frequency.
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Cumulative transcription factor binding and p300 activity determine enhancer activation frequency in eukaryotic cells.
- Why it matters: Understanding how often enhancers are active in individual cells is crucial for decoding gene regulation variability and cellular responses, yet the underlying mechanisms remain unclear.
- What they did: Using single-molecule footprinting, the study quantified chromatin accessibility at enhancers in response to TF perturbations and chromatin changes, analyzing hundreds of enhancers at ectopic sites.
- The result: Findings reveal that multiple TFs collectively control enhancer activation frequency and that p300 activity is essential for full enhancer activation, enabling targeted modulation of gene expression.