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Cumulative transcription factor binding and p300-mediated histone acetylation drive enhancer activation frequency.
Nature Genetics · · Journal Article
Baderna, Barzaghi + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Cumulative transcription factor binding and p300 activity determine enhancer activation frequency in eukaryotic cells.
- Why it matters: Understanding how often enhancers are active in individual cells is crucial for decoding gene regulation variability and cellular responses, yet the underlying mechanisms remain unclear.
- What they did: Using single-molecule footprinting, the study quantified chromatin accessibility at enhancers in response to TF perturbations and chromatin changes, analyzing hundreds of enhancers at ectopic sites.
- The result: Findings reveal that multiple TFs collectively control enhancer activation frequency and that p300 activity is essential for full enhancer activation, enabling targeted modulation of gene expression.
The findingWhy it mattersWhat they didThe result
- 3 cites