Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening.

PRIME-VLP enhances prime editing efficiency by up to 2.9-fold across diverse targets using multiple sequential virus-like particle transductions.

• Why it matters: Improving prime editing efficiency is crucial for precise genome modifications and high-throughput functional genomics, yet current methods face limitations in effectiveness and scalability.
• What they did: Researchers developed PRIME-VLP, which employs repeated sub-saturating eVLP transductions and decouples pegRNA from the editor, enabling pooled screens with a 6,000-pegRNA library targeting TP53.
• The result: This approach significantly boosts editing accuracy and reproducibility without increasing off-target effects, paving the way for advanced genome editing applications and functional genomics studies.