Optimised genome editing for precise DNA insertion and substitution using prime editors in zebrafish.

Prime editors achieve up to 27.3% precise DNA insertions in zebrafish, enabling accurate genome modifications without exogenous DNA.

• Why it matters: Precise genome editing is essential for functional studies and disease modeling, but current methods like CRISPR/Cas9 often result in random insertions and deletions, limiting accuracy.
• What they did: The study employed nickase- and nuclease-based prime editors to perform targeted short DNA substitutions and insertions at multiple zebrafish loci, including longer fragment insertions.
• The result: Prime editing demonstrated high efficiency and heritability of modifications, providing a powerful tool for precise genetic manipulation in zebrafish without external DNA donors.