Integrated in vitro multi-endpoint framework for assessing 6PPD-Quinone cytotoxicity in fish and mammalian cell lines.

Fish-derived cell lines exhibit greater susceptibility to 6PPD-Q toxicity than mammalian cells, with pNPA values indicating higher phenotypic perturbations across five cell types.

• Why it matters: Understanding the differential toxicity of 6PPD-Q across species is crucial for ecological risk assessment, especially given its widespread environmental presence and toxicity concerns.
• What they did: The study employed integrated cellular and phenotypic profiling, including viability, apoptosis, cell cycle, and morphological analyses, across five cell lines, and used two scoring frameworks—pNPA and RBIF—to quantify toxicity.
• The result: Findings reveal tissue-origin influences susceptibility and provide a quantitative in vitro framework for comparing phenotypic effects, aiding future toxicity evaluations, though environmental relevance requires cautious interpretation.