Expanding the scope of precision editing in seaweeds through the application of a novel CRISPR-associated nuclease 12a-aligned CRISPR system in Ulva prolifera.

A novel Cas12a-aligned CRISPR system enabled over 50 gene knockouts in Ulva prolifera, expanding precision genome editing in seaweeds for the first time.

• Why it matters: Seaweeds like Ulva prolifera are promising marine crops, but limited genome-editing tools hinder functional studies and industrial applications, creating a critical gap in algal biotechnology.
• What they did: Researchers optimized delivery conditions and compared Cas systems, successfully applying a transgene-free, AT-rich-targeting CRISPR-associated protein to generate knockout mutants at 28°C.
• The result: This work broadens genome-editing options in seaweeds, facilitating functional genomics and enabling targeted exploration of previously inaccessible algal resources.