From fragmented workflows to integrated pipelines: Bridging enzymatic DNA synthesis, assembly, and MutS-based error correction.

Integrating enzymatic DNA synthesis, assembly, and MutS-based error correction into a continuous pipeline enhances fidelity and scalability for gene construction.

• Why it matters: Current workflows are fragmented, limiting the accuracy, throughput, and scalability of constructing large DNA sequences, which hampers progress in synthetic biology.
• What they did: The authors analyze error propagation during synthesis and assembly, compare correction strategies, and highlight enzymatic DNA synthesis's potential to enable an integrated, automated process.
• The result: This integrated pipeline improves error correction efficiency and workflow compatibility, paving the way for high-fidelity, high-throughput gene and pathway-scale DNA construction.