Short-read whole-genome sequencing identifies the Tg(Tal1-cre/ERT)42-056Jrg transgene insertion site on mouse chromosome 4

Short-read whole-genome sequencing mapped the Tg(Tal1-cre/ERT)42-056Jrg (HSC-Scl-CreERT) transgene insertion site to mouse chromosome 4, about 6 kb upstream of Echdc2.

• Why it matters: Knowing the exact genomic landing site of a widely used hematopoietic CreERT allele helps interpret experiments and control for insertion-context effects.
• What they did: The insertion locus was determined using short-read whole-genome sequencing, followed by localization relative to nearby annotated genes on chromosome 4.
• The result: The identified insertion position provides a reference for downstream analyses and for designing or comparing studies that use this specific transgenic line.

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