Compartment-Resolved Matrisome Shifts During Postnatal Tendon Maturation in Mice

Sequential extraction LC-MS/MS shows distinct matrisome trajectories in murine Achilles and plantaris tendons from P0 to P28, with insoluble collagenous signal rising from 95% to over 99%.

• Why it matters: Tendon load-bearing capacity emerges during the first postnatal month, but a comprehensive, compartment-level view of how the matrisome remodels during this window is missing.
• What they did: Compartment-resolved proteomics used a CHAPS-soluble fraction and an insoluble mature ECM fraction solubilized with guanidine hydrochloride and hydroxylamine hydrochloride, analyzed by label-free quantitative LC-MS/MS at P0, P14, and P28.
• The result: The insoluble matrix composition is largely established by P14, while the soluble compartment shows collagen increasing from 50% to over 60% by P14 alongside dynamic regulation of collagen processing, crosslinking, fibril assembly regulators, and additional fibril-associated proteoglycans, matricellular proteins, and angiogenic regulators.

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