Fallopian Tube Organoid-Integrated Microfluidic Platform for Real-Time Imaging and Outflow Analysis

A PDMS microfluidic platform integrating mechanically opened fallopian tube organoids forms luminal-like epithelial sheets covering >90% of channel area by day 6 (n=5).

• Why it matters: Existing 2D, intact organoid, and static Transwell models do not recapitulate a polarized epithelium lining a perfused lumen under physiological shear while preserving native organoid secretory function.
• What they did: Organoid fragments were integrated into a custom PDMS device to re-establish luminal-like architecture under controlled perfusion, enabling longitudinal imaging and ELISA-compatible collection of conditioned flow-through for up to 10 days.
• The result: The epithelial sheets formed continuous ZO-1-positive tight junctions, maintained on-chip health comparable to intact organoids with IL1 alpha undetectable in chip outflow, and computational flow modelling predicted distribution of secreted factors.