OTTER: Orchestrated Transcriptomic, Tumor-xenograft (PDX/CDX), and Epigenomic Reporting Workflow

OTTER restores bisulfite sequencing graft recall to 52%-54% across a 0%-99% compositional gradient in tumor xenograft libraries with infiltrating murine host reads.

• Why it matters: Conventional deconvolution tools fail on BS-seq because C-to-T deamination collapses sequence entropy, destroying graft recall (< 0.015%).
• What they did: OTTER is an automated multi-omics workflow that uses static domain operators, an immutable reference registry, and a native strand-aware edit-distance engine for bisulfite deconvolution.
• The result: The workflow preserves complete decision parity on transcriptomics while enabling bisulfite deconvolution with 52%-54% recall, and it is available open-source for x86_64 Linux.