Mitochondrial and bioenergetic processes are enriched in large-but are reciprocally depleted in small extracellular vesicles

Mitochondrial and bioenergetic processes are enriched in brain endothelial cell–derived large extracellular vesicles (>200 nm) but reciprocally depleted in small EVs (<200 nm).

• Why it matters: EVs are promising drug-delivery carriers, but cargo selectivity by EV size is unclear, limiting mechanistic design for therapies targeting mitochondrial dysfunction.
• What they did: Size-resolved quantitative proteomics with gene-set enrichment analysis compared large EVs (>200 nm) versus small EVs (exosomes, <200 nm) from brain endothelial cells.
• The result: The data indicate selective packaging of mitochondrial cargo in large EVs but not small EVs, supporting continued development of large EV mitochondria for diseases linked to mitochondrial dysfunction.