Comparative Multi-omics Reveals Conserved Membrane Architecture and Selective Metabolic Cargo Enrichment Across Yeast-Derived Extracellular Vesicles

Yeast-derived extracellular vesicles from Saccharomyces cerevisiae, Rhodotorula toruloides, and two Yarrowia lipolytica strains preserve conserved bilayer membrane architecture while selectively enriching metabolically active cargo.

• Why it matters: EV biology has been characterized mainly in mammalian systems, leaving yeast-derived EVs poorly defined despite their potential as scalable, non-pathogenic platforms for biotechnology and metabolic engineering.
• What they did: The study used tangential flow filtration with ultracentrifugation to isolate intact, bilayer-enclosed YDEVs from three yeast species and four strains, then applied comparative multi-omics (metabolomics and lipidomics).
• The result: NAD was enriched in SC and RT-88 EVs but nearly absent from Yarrowia-derived vesicles, while lipidomics showed broadly conserved membrane architecture with quantitative remodeling of lipid classes and unsaturation profiles.