OpenAntigens: a structure-aware database for antigen construct design across the human cell-surface and secreted proteome.

OpenAntigens provides 55 305 construct suggestions and 148 722 sequence-similarity hits for 5328 human secreted and membrane proteins, enabling structure-aware antigen construct design.

• Why it matters: Antibody discovery, recombinant reagents, structural biology, and binder design need standardized choices of protein regions, but topology, processing, structures, modifications, partners, and cross-reactivity risk are hard to reconcile.
• What they did: The free, no-login database integrates UniProt topology, AlphaFold pLDDT and PAE, PDB precedent, domain annotations, orthologs/paralogs, disease associations, partner context, and BLAST to generate construct-design reports.
• The result: For targets with compatible AlphaFold models, an interactive designer links sequence, structure, pLDDT, and PAE to revise boundaries and export species-equivalent sequences with real-time cysteine and modification warnings.