Nat Cell BiolJClub
Tubulin acetylation regulates nuclear shape fluctuations and DNA repair.
Nature Cell Biology · · Journal Article · Open access
Wallis, Tse + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
DNA damage induces progressive tubulin acetylation that displaces the perinuclear vimentin cage, enabling transient nuclear shape fluctuations linked to homologous recombination repair.
- Why it matters: Genome stability relies on accurate DSB repair, but how nuclear mechanics shape repair outcomes is unclear.
- What they did: The study tracked DNA damage–driven tubulin acetylation, vimentin cage displacement, nuclear mechanics, DSB mobility, and HR versus NHEJ after inhibiting tubulin acetylation.
- The result: Inhibiting tubulin acetylation abolishes nuclear fluctuations, restricts DSB mobility, and negatively impacts homology search during HR while leaving NHEJ intact, increasing persistent DSB clustering, cell-cycle arrest, senescence, and sensitivity to DNA-damaging agents; removing the vimentin cage rescues fluctuations and repair efficiency.
The findingWhy it mattersWhat they didThe result
- Open access