Plant Physiol BiochemJClub
BrABA2 mutation conferred glossy leaf via epicuticular wax crystal disruption in Chinese cabbage.
Plant Physiology and Biochemistry · · Journal Article · Open access
Zhao, Song + more
Abstract ↗AI summary
The abstract is read at the publisher; the summary is JClub's.
Mutation of BrABA2 in Chinese cabbage (Brassica rapa ssp. pekinensis) causes the glossy leaf phenotype by disrupting epicuticular wax crystal formation on leaf surfaces.
- Why it matters: Leaf glossiness strongly affects commercial value, yet the genes controlling this trait in Chinese cabbage remain unclear for breeding and improvement.
- What they did: An EMS-mutagenized doubled haploid line ‘FT’ produced the glossy mutant wdm19; SEM, water loss/chlorophyll leaching, GC-MS wax profiling, MutMap, and KASP genotyping identified BrABA2 (BraA08g002170.3.5C).
- The result: A G-to-A substitution 304 bp downstream of the TAG stop codon in BrABA2 3' UTR reduced transcript and protein expression, and Arabidopsis complementation restored the aba2 phenotype, linking BrABA2 loss to glossiness.
The findingWhy it mattersWhat they didThe result
- Open access