RNAffinityDB: a curated database of experimental RNA-protein binding affinities.

RNAffinityDB compiles 10,016 curated experimental RNA-protein binding affinity records from over 1,000 publications, linking 3,057 protein constructs and 4,638 RNA sequences.

• Why it matters: Understanding how sequence determinants influence RNA-protein binding strength is hindered by fragmented and inconsistent data, limiting systematic analysis and model development.
• What they did: The database was created by anchoring each measurement to specific protein constructs and RNA sequences, preserving affinity values, measurement boundaries, and non-binding observations, with a web interface for retrieval and analysis.
• The result: RNAffinityDB enables systematic analysis of sequence-affinity relationships and supports the development of RNA-protein affinity models, facilitating advances in understanding RNA-protein interactions.