Integrated in vitro transcription and oligo-dT affinity chromatography enable multi-cycle reagent recycling for mRNA manufacturing.

Multi-cycle reagent recycling in in vitro transcription and purification reduces raw-material costs by over twofold while maintaining RNA quality over five cycles.

• Why it matters: High operational costs hinder scalable, affordable production of RNA-based vaccines and therapeutics, emphasizing the need for more efficient manufacturing processes.
• What they did: An integrated sequential-batch IVT-oligo-dT chromatography process was developed, linking synthesis and purification with shared buffers to enable reagent reuse across five cycles at an 8 mL scale.
• The result: Recycling improved 5' cap analog utilization by 3.72 times and cost efficiency by 2.21 times, with stable RNA integrity, reduced dsRNA content, and consistent biological activity, supporting more sustainable mRNA manufacturing.